Leiden Muscular Dystrophy pages©

alpha-Sarcoglycan (SGCA) primers

(last modified November 28, 2004)

Contents

DNA-primers

Exon PCR

Exon Length Reference forward primer reverse primer Name
1   Piccolo gtgtctatcccagatttgg accacccctaaatcctgcc  
2 + 3   Piccolo accattaatagggggcctaa ctcaatgacctgga  
3 - 5   Roberds ggctcctgctgtgactcgaatc tagccagaggagagtggtga  
4 + 5   Piccolo ggcctcctaggagcagccctatg gacccttgttctatgcccgcc  
6   Piccolo ccgcttcctctcagccttgg atccaccaagaagtcacggt  
7   Piccolo tgtgcgctaaccagtgca agtgccactgtgagtccc  
8   Piccolo caggagggcattgtggat gcatcaccctggagttat  
9   Piccolo ggaacacagaggagctgcgg tgtggacagagaagggaggatg  

Legend:
Exonic sequences are in upper case, intronic and gene flanking sequences in lower case and added primer tails in italics. Amplified: region amplified. Numbering of exons is according to Roberds et al. (1994). Length (in bp): length of PCR-product in basepairs. Forward primer / reverse primer: sequence of forward and reverse primer. Name: name of the primers. Reference: publication describing the primer(s).

DGGE

Exon Length
(in bp)		 Forward primer /
 reverse primer		 DGGE-
gradient		 Name Reference
1 128 cctgtctctgtcactcaccg /
PSO-aatcctgccccctcatcctgcc		 40-90 Ex1F/R Carrié
2 197 gcctgtgtgtttgggacttg /
PSO-aatacaccccagcccgcccgcc		 40-90 Ex2F/R Carrié
3 239 gggctcctgctgtgactcga /
PSO-aatggcccacccctgtgatttt		 50-90 Ex3F/R Carrié
4 162 PSO-aatttctcccctaacccacttc /
aagactggcattggtgggga		 40-90 Ex4F/R Carrié
5 311 PSO-aagcagggatgtggggaggagc /
agtcctctagggttgcacac		 40-90 Ex5F/R Carrié
6 444 ctgcccccactctgctgaca /
PSO-aagggaagggggactgggatgg		 30-80 and
40-90 (after MboII digestion)		 Ex6F/R Carrié
7 339 tgtccagccagccacttcct /
PSO-aatcccatacagacaagtgcca		 40-90 Ex7F/R Carrié
8 152 PSO-aagggcaccttggggcgaaacc /
atagtttggtggcggggtcc		 30-80 Ex8F/R Carrié

Legend:
Exon: exon number. Length (in bp): length of PCR fragment (in base pair). Forward primer / reverse primer: sequence of PCR forward and reverse primer (5' to 3'). PSO-=psoralen clamp. DGGE-gradient: gradient used during Denaturing Gradient Gel Electrophoresis. Name: name of the primers. Reference: publication describing the primer(s).

DHPLC

DHPLC-analysis of the SGCA-gene, have also been published.

RNA-primers

A six-pair of primers to amplify alpha-sarcoglycan mRNA has also been reported by Kawai et al. (1995)

Exon Length
(in bp)		 Nucleotides Reference Forward primer /
reverse primer		 Name
. - 5 ... ...- 483 Carrié CCGTGTCTTTGTGCACACCTTGGA /
CAAGGCTGAGAGGAAGCGGCTGGCA		 set1F/R
1 - 4 355 -35 - 317 Mongini TCTGTCACTCACCGGGCGG /
GTGACCTCAATGACCTGGAG		 1F/R
1 - 4 352 -36 - 315 Ljunggren primers improved by Mongini (2002)
CTCTGTCACTCACCGGC /
GACCTCAATGACCTGGAGC		 H1F/R
1 - 6 695 -35 - 650 Passos-Bueno TCTGTCACTCACCGGCGG /
TCGGGGGATGCCACCATC		 r.ex1F/6R
3 - 6 339 273 - 611 Ljunggren CTCTGCCACCCCAGAAGAT /
GAGGCAGAACCCACCTTAAT		 H2F/R
5 - 9 655 465 - 1119 Carrié CCGCTTCCTCTCAGCCTTGG /
GGGAGGCAGCCGCTCACCTGTG		 set2F/R
5 - 8 480 514 - 993 Ljunggren CTGCTCAACGTCACCTCTG /
CATCTGGATGTCGGAGGTAG		 H3F/R
6 - 10 671 572 - 1241 Passos-Bueno GCCGAAAAGAAGGGGTATAC /
TGGGATGTGCCACTCGTG		 r.ex6F/10R
 8 - 10 426 950 - 1375 Ljunggren AGGGAAGGCTGAAGAGAGAC /
CAGCAATTGGTGAGCAGAG		 H4F/R
9 - 10 205 1018 - 1222 Carrié AACACAGAGGAGCTGCGG /
TGTGGACAGAGAAGGGAGGATG		 set32F/R

Legend:
Exon: exon number. Numbering of exons is according to Roberds et al. (1994). Length (in bp): length of PCR-product in basepairs. Nucleotides: nucleotides amplified (numbering according to the cDNA Reference Sequence). Forward primer / reverse primer: sequence of forward / reverse primer. Name: name of the primers. Reference: publication describing the primer(s).

| Top of page | LMDp homepage |
| Sarcoglycan homepage | SGCA, SGCB, SGCG, SGCD SGCE, SGCZ |
| Remarks / information | Copyright©, liability |