Leiden Muscular Dystrophy pages©

alpha-Sarcoglycan (SGCA) primers

(last modified November 28, 2004)


Contents



DNA-primers

Exon PCR

Exon Length Reference forward primer reverse primer Name
1   Piccolo gtgtctatcccagatttgg accacccctaaatcctgcc  
2 + 3   Piccolo accattaatagggggcctaa ctcaatgacctgga  
3 - 5   Roberds ggctcctgctgtgactcgaatc tagccagaggagagtggtga  
4 + 5   Piccolo ggcctcctaggagcagccctatg gacccttgttctatgcccgcc  
6   Piccolo ccgcttcctctcagccttgg atccaccaagaagtcacggt  
7   Piccolo tgtgcgctaaccagtgca agtgccactgtgagtccc  
8   Piccolo caggagggcattgtggat gcatcaccctggagttat  
9   Piccolo ggaacacagaggagctgcgg tgtggacagagaagggaggatg  

Legend:
Exonic sequences are in upper case, intronic and gene flanking sequences in lower case and added primer tails in italics. Amplified: region amplified. Numbering of exons is according to Roberds et al. (1994). Length (in bp): length of PCR-product in basepairs. Forward primer / reverse primer: sequence of forward and reverse primer. Name: name of the primers. Reference: publication describing the primer(s).


DGGE

Exon Length
(in bp)
Forward primer /
 reverse primer
DGGE-
gradient
Name Reference
1 128 cctgtctctgtcactcaccg /
PSO-aatcctgccccctcatcctgcc
40-90 Ex1F/R Carrié
2 197 gcctgtgtgtttgggacttg /
PSO-aatacaccccagcccgcccgcc
40-90 Ex2F/R Carrié
3 239 gggctcctgctgtgactcga /
PSO-aatggcccacccctgtgatttt
50-90 Ex3F/R Carrié
4 162 PSO-aatttctcccctaacccacttc /
aagactggcattggtgggga
40-90 Ex4F/R Carrié
5 311 PSO-aagcagggatgtggggaggagc /
agtcctctagggttgcacac
40-90 Ex5F/R Carrié
6 444 ctgcccccactctgctgaca /
PSO-aagggaagggggactgggatgg
30-80 and
40-90 (after MboII digestion)
Ex6F/R Carrié
7 339 tgtccagccagccacttcct /
PSO-aatcccatacagacaagtgcca
40-90 Ex7F/R Carrié
8 152 PSO-aagggcaccttggggcgaaacc /
atagtttggtggcggggtcc
30-80 Ex8F/R Carrié

Legend:
Exon: exon number. Length (in bp): length of PCR fragment (in base pair). Forward primer / reverse primer: sequence of PCR forward and reverse primer (5' to 3'). PSO-=psoralen clamp. DGGE-gradient: gradient used during Denaturing Gradient Gel Electrophoresis. Name: name of the primers. Reference: publication describing the primer(s).


DHPLC

DHPLC-analysis of the SGCA-gene, have also been published.


RNA-primers

A six-pair of primers to amplify alpha-sarcoglycan mRNA has also been reported by Kawai et al. (1995)


Exon Length
(in bp)
Nucleotides Reference Forward primer /
reverse primer
Name
. - 5 ... ...- 483 Carrié CCGTGTCTTTGTGCACACCTTGGA /
CAAGGCTGAGAGGAAGCGGCTGGCA
set1F/R
1 - 4 355 -35 - 317 Mongini TCTGTCACTCACCGGGCGG /
GTGACCTCAATGACCTGGAG
1F/R
1 - 4 352 -36 - 315 Ljunggren primers improved by Mongini (2002)
CTCTGTCACTCACCGGC /
GACCTCAATGACCTGGAGC
H1F/R
1 - 6 695 -35 - 650 Passos-Bueno TCTGTCACTCACCGGCGG /
TCGGGGGATGCCACCATC
r.ex1F/6R
3 - 6 339 273 - 611 Ljunggren CTCTGCCACCCCAGAAGAT /
GAGGCAGAACCCACCTTAAT
H2F/R
5 - 9 655 465 - 1119 Carrié CCGCTTCCTCTCAGCCTTGG /
GGGAGGCAGCCGCTCACCTGTG
set2F/R
5 - 8 480 514 - 993 Ljunggren CTGCTCAACGTCACCTCTG /
CATCTGGATGTCGGAGGTAG
H3F/R
6 - 10 671 572 - 1241 Passos-Bueno GCCGAAAAGAAGGGGTATAC /
TGGGATGTGCCACTCGTG
r.ex6F/10R
 8 - 10 426 950 - 1375 Ljunggren AGGGAAGGCTGAAGAGAGAC /
CAGCAATTGGTGAGCAGAG
H4F/R
9 - 10 205 1018 - 1222 Carrié AACACAGAGGAGCTGCGG /
TGTGGACAGAGAAGGGAGGATG
set32F/R

Legend:
Exon: exon number. Numbering of exons is according to Roberds et al. (1994). Length (in bp): length of PCR-product in basepairs. Nucleotides: nucleotides amplified (numbering according to the cDNA Reference Sequence). Forward primer / reverse primer: sequence of forward / reverse primer. Name: name of the primers. Reference: publication describing the primer(s).



| Top of page | LMDp homepage |
| Sarcoglycan homepage | SGCA, SGCB, SGCG, SGCD SGCE, SGCZ |
| Remarks / information | Copyright©, liability |