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FKRP amplification primers

( LGMD-2I / MDC1C )

(last modified January 13, 2005)

DNA
- gene
- CA-repeats
RNA

DNA-primers - gene

Amplified	Location	Length (in bp)	Reference	forward primer	reverse primer	Name
exon 1	-867 - -267	601	Walter	ttacacagccctccttccg	gccgccatcttggagcaat	PRO 1S/1R
exon 4	-39-131 - 232	403	Louhichi	caaagctgaaaccaaataggga	GCTGGGCTGGGTCTTGCTG	FKRP 4-1
exon 4	-39-105 - ...	1,697	Brockington	aaagggaattgagaaagagc	GCTCACACAGAGCTTCTCC	FKRP-1F/5R
exon 4	-39-105 - 214	281	Brockington, Walter	aaagggaattgagaaagagc	GCAGGAAGGAGTCTACCAG	FKRP-1F/1R
exon 4	137 - 545	409	Louhichi	GCCCCCGTGTCACCGTCCT	TAGCGGGCGGTCCACTCTC	FKRP 4-2
exon 4	167 - 634	468	Brockington, Walter	TCGAGGCATTTGACAACGCGG	CCGAGAGGTTGAAGAGGT	FKRP-2F/2R
exon 4			Brockington		CTCCTCGTAGAGGTAGGC	FKRP-3R
exon 4	375 - 885	511	Louhichi	TGTGGCCCTAGTACCTGATG	GCGCGTGGTCTCCTTGTTG	FKRP 4-3
exon 4	658 - 870	213	Walter	ACCAGCCTCTTTCTGCAGACC	TGGAGTGGTTCGGCTGCAAC	FKRP-3F/3R
exon 4	827 - 1272	446	Louhichi	TAGTGAGCTGGGAAGGCGG	ATTGCGGGGGTAGAAGGG	FKRP 4-4
exon 4	1099 - 1189	91	Brockington, Walter	ATCTACTTGGAGGACGTGGGCAA	CCTTCTCCCATACGAAGC	FKRP-4F/4R
exon 4	1215 - 1636	421	Louhichi	GCAGTACAGCGAAAGCAACC	GCCTTCTCTCATGCTCTCCT	FKRP 4-5
exon 4 826C>A mutation		573	Brockington	ACCTCTTCAACCTCTCGG^*	CCTTCTCCCATACGAAGC	FKRP-2F/4R (digest with BfaI,yielding 400 and 500 bp fragments)

Legend:
Exonic sequences are in upper case, intronic and gene flanking sequences in lower case and added primer tails in italics. Amplified: region amplified; numbering of exons is according to Brockington. Location: region amplified, nucleotide numbering according to the coding DNA reference sequence. Length: length of PCR-product in basepairs. Reference: publication describing the primer(s). Forward primer: sequence of forward primer. Reverse primer: sequence of reverse primer. Name: name of the primers.
^*NOTE: sequence of this primer was published as reverse (Brockington).

CA-repeats

Marker	Length (in bp)	Location (in Mb)	Forward / reverse primer	Reference
D19S219 (AFM210yg9)	174	+1.26	gtgagccaagattgtgcc / gactatttctgagacagattccca	GenBank Z23600
D19S412 (AFM284yg5)	113	+0.24	tgagcgacagaatgagact / acatcttactgaatgcttgc	GenBank Z24042
fkrp52	92 - 122 (120 = CA₂₆)	+0.04	tctccaaaaaacaacaacaac / ctagtgttctgggaccttt	Louhichi [2004]
FKRP		0	ccaacacactgtctgcctt
D19S606 (AFMb030we1)	178 (= AC₂₀)	-0.72	agggctgggacctca / ccaacacactgtctgcctt	GenBank Z53152

Legend:
Marker: name of DNA CA-repeat marker (with link to GDB). Length (in bp): length of PCR-product in base pairs. Forward / reverse primer: sequence of forward and reverse primer. Reference: publication describing the primer(s). Name: name of the primers.

RNA-primers

Amplified	Length	Reference	Forward primer	Reverse primer	Name

Legend:
Exonic sequences are in upper case, intronic and gene flanking sequences in lower case and added primer tails in italics. Amplified: region amplified. Numbering of exons is according to.... Length: length of PCR-product in basepairs. Reference: publication describing the primer(s). Forward primer: sequence of forward primer. Reverse primer: sequence of reverse primer. Name: name of the primers.