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SGCA sequences (for DHPLC)

(last modified on November 30, 2002)

Contributed by Don Love - d.love @ auckland.ac.nz

DHPLC analysis of the sarcoglycan genes

Exon 1 (160 bp)

cttccctcct ctcctccctg ccccctgtCT CTGTCACTCA CCGGGCGGGC CAGGCCGGGC 
AGCCATGGCT GAGACACTCT TCTGGACTCC TCTCCTCGTG Ggcaagttgg ggccttgttc 
agcggggagg cccaggatga gggggcagga tttaggggtg

Exon 2 (240 bp)

agcttatccc ctgcccagga ctgaggctgg cctgtgtgtt tgggacttgt ggggtcccca 
cagTTCTCCT GGCAGGGCTG GGGGACACCG AGGCCCAGCA GACCACGCTA CACCCACTTG 
TGGGCCGTGT CTTTGTGCAC ACCTTGGACC ATGAGACGTT TCTGAGCCTT CCTGAGCATG 
TCGgtgagcg gcctgacagg cacccaggcg ggcgggctgg ggtgtacccc gcagggctcc

Exon 3 (270 bp)

gcagggctcc tgctgtgact cgaatcccct ctcctcgctt ccaccagCTG TCCCACCCGC 
TGTCCACATC ACCTACCACG CCCACCTCCA GGGACACCCA GACCTGCCCC GGTGGCTCCG 
CTACACCCAG CGCAGCCCCC ACCACCCTGG CTTCCTCTAC GGCTCTGCCA CCCCAGAAGA 
TCGTGGGCTC CAGGTCATTG AGgtgccgtc agggaccctg agaaaatcac aggggtgggc 
cagagtggcc tcctaggagc agccctatga

Exon 4 (180 bp)

gtgccacgtt tctcccctaa cccacttctc agatgtcttt cccatccccc agGTCACAGC 
CTACAATCGG GACAGCTTTG ATACCACTCG GCAGAGGCTG GTGCTGGAGA TTGGGGACCC 
AGAAGgtacc tctagctgtg ccccatccct tccccaccaa tgccagtctt ggggaatctc

Exon 5 (329 bp)

cttcaaggag gctttgcggg gcagagctgg ggctgggtgc agcctgaggt gtccacctgg
ccttcccagG CCCCCTGCTG CCATACCAAG CCGAGTTCCT GGTGCGCAGC CACGATGCGG
AGGAGGTGCT GCCCTCAACA CCTGCCAGCC GCTTCCTCTC AGCCTTGGGG GGACTCTGGG
AGCCCGGAGA GCTTCAGCTG CTCAACGTCA CCTCTGCCTT GGACCGTGGG GGCCGTGTCC
CCCTTCCCAT TGAGGGCCGA AAAGAAGgta ggtgtgcaac cctagaggac ttcctgaaag
aggaggatgc agcttgtgg cgggcataga

Exon 6 (311 bp)

ttcaacaacc cctggcagag tggtgcctcg tgccccctgc ccccactctg ctgacagtga 
cttctatctg gtcccagGGG TATACATTAA GGTGGGTTCT GCCTCACCTT TTTCTACTTG
CCTGAAGATG GTGGCATCCC CCGATAGCCA CGCCCGCTGT GCCCAGGGCC AGCCTCCACT
TCTGTCTTGC TACGACACCT TGGCACCCCA CTTCCGCGTT GACTGGTGCA ATGTGACCCT
Ggtgaggagg gaccctgggt ccgggggtgg ggtggggcat ggcccccatc ccagtccccc
ttccctccca t

Exon 7 (320 bp)

tgtgtccagc cagccacttc ctgcgtcagc cctgagctct ctgtgcagGT GGATAAGTCA 
GTGCCGGAGC CTGCAGATGA GGTGCCCACC CCAGGTGATG GGATCCTGGA GCATGACCCG 
TTCTTCTGCC CACCCACTGA GGCCCCAGAC CGTGACTTCT TGGTGGATGC TCTGGTCACC 
CTCCTGGTGC CCCTGCTGGT GGCCCTGCTT CTCACCTTGC TGCTGGCCTA TGTCATGTGC 
TGCCGGCGGG AGGGAAGgtg aatgtgggca tgaagggcgg gggagcacct gctggagctc 
acacccatgg gactcacagt

Exon 8 (119 bp)

agagctgggc taaccctctc cttcactttt ccacagGCTG AAGAGAGACC TGGCTACCTC
CGAgtgagta aaggaaagct gggggtgggg tgggagccca cctagacagt tgttggacc

Exon 9 (320 bp)

tccaggctgt actccagggc agctgactcc cagagctgat gactccccac ctgtgcctcc 
agCATCCAGA TGGTCCACCA CTGCACCATC CACGGGAACA CAGAGGAGCT GCGGCAGATG 
GCGGCCAGCC GCGAGGTGCC CCGGCCACTC TCCACCCTGC CCATGTTCAA TGTGCACACA 
GGTGAGCGGC TGCCTCCCCG CGTGGACAGC GCCCAGGTGC CCCTCATTCT GGACCAGCAC 
TGACAGCCCA GCCAGgtagg tctggtgggt gatgccagcc ttatttctgg gaacaagatg 
gcctttatca caaggctggg

Exon 10 (340 bp)

ggaagtcagg agaaccagca tctggccctc tcagccctcc actctctctt cccacagTGG 
TTCCAGGTCC AGCCCTGACT TCATCCTCCC TTCTCTGTCC ACACCACGAG TGGCACATCC 
CACCTGCTGA TTCCAGCTCC TGGCCCTCCT GGAACCCAGG CTCTAAACAA GCAGGGAGAG 
GGGGTGGGGT GGGGTGAGAG TGTGTGGAGT AAGGACATTC AGAATAAATA TCTGCTGCTC 
TGCTCACCAA TTGCTGCTGG CAGCCTCTCC CGTCctcact ggctctgttt ccctttgtgc 
tttctgggag gccccatagg cagccagtgc ctgccggcct

Legend:
Sequences indicated in blue represent the primers used to generate the amplicons for DHPLC analysis. Sequences in red represent exonic sequences, while those in black represent intronic and/or gene flanking sequences. Sequences indicated in green and underlined represent the translation start and stop codons and the polyA-addition signal.

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